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psectag 2b expression plasmid  (Thermo Fisher)


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    Structured Review

    Thermo Fisher psectag 2b expression plasmid
    Psectag 2b Expression Plasmid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psectag+2b+expression+plasmid/psectag+2b+expression+plasmid/pmc08133003-78-15-25
    Average 90 stars, based on 1 article reviews
    psectag 2b expression plasmid - by Bioz Stars, 2026-09
    90/100 stars

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    Polymerase Chain Reaction:

    Article Title: Alternative splicing of the metalloprotease ADAMTS17 spacer regulates secretion and modulates autoproteolytic activity
    Article Snippet: .. The PCR products were digested with HindIII × XhoI and ligated in the HindIII × XhoI digested pSecTag 2B expression plasmid (ThermoFisher Scientific) using T4 DNA ligase (New England Biolabs). ..

    Article Title: Alternative splicing of the metalloprotease ADAMTS17 spacer regulates secretion and modulates autoproteolytic activity
    Article Snippet: 28 Additional ADAMTS17 constructs were cloned by generating the following PCR products using ADAMTS17B as template and the Phusion High Fidelity DNA polymerase (ThermoFisher Scientific) (forward/reverse primer pairs indicated in parentheses, Hind III and Xho I restriction sites are underlined): 1CS (5′-at aagctt tggagcccgtggggcgcc-3′/5′-at ctcgag cgatgaacaaagagtcctg-3′), S (5′-at aagctt cacttggtgaagggcgac-3′/5′-at ctcgag cgatgaacaaagagtcctg-3′), and DC (5′-at aagctt ttgctagtcacggacccc-3′/5′-at ctcgag cgcaggtcttgccgtcccc-3′). .. The PCR products were digested with Hind III × Xho I and ligated in the Hind III × Xho I digested pSecTag 2B expression plasmid (ThermoFisher Scientific) using T4 DNA ligase (New England Biolabs). ..

    Expressing:

    Article Title: Alternative splicing of the metalloprotease ADAMTS17 spacer regulates secretion and modulates autoproteolytic activity
    Article Snippet: .. The PCR products were digested with HindIII × XhoI and ligated in the HindIII × XhoI digested pSecTag 2B expression plasmid (ThermoFisher Scientific) using T4 DNA ligase (New England Biolabs). ..

    Article Title: Alternative splicing of the metalloprotease ADAMTS17 spacer regulates secretion and modulates autoproteolytic activity
    Article Snippet: 28 Additional ADAMTS17 constructs were cloned by generating the following PCR products using ADAMTS17B as template and the Phusion High Fidelity DNA polymerase (ThermoFisher Scientific) (forward/reverse primer pairs indicated in parentheses, Hind III and Xho I restriction sites are underlined): 1CS (5′-at aagctt tggagcccgtggggcgcc-3′/5′-at ctcgag cgatgaacaaagagtcctg-3′), S (5′-at aagctt cacttggtgaagggcgac-3′/5′-at ctcgag cgatgaacaaagagtcctg-3′), and DC (5′-at aagctt ttgctagtcacggacccc-3′/5′-at ctcgag cgcaggtcttgccgtcccc-3′). .. The PCR products were digested with Hind III × Xho I and ligated in the Hind III × Xho I digested pSecTag 2B expression plasmid (ThermoFisher Scientific) using T4 DNA ligase (New England Biolabs). ..

    Plasmid Preparation:

    Article Title: Alternative splicing of the metalloprotease ADAMTS17 spacer regulates secretion and modulates autoproteolytic activity
    Article Snippet: .. The PCR products were digested with HindIII × XhoI and ligated in the HindIII × XhoI digested pSecTag 2B expression plasmid (ThermoFisher Scientific) using T4 DNA ligase (New England Biolabs). ..

    Article Title: Alternative splicing of the metalloprotease ADAMTS17 spacer regulates secretion and modulates autoproteolytic activity
    Article Snippet: 28 Additional ADAMTS17 constructs were cloned by generating the following PCR products using ADAMTS17B as template and the Phusion High Fidelity DNA polymerase (ThermoFisher Scientific) (forward/reverse primer pairs indicated in parentheses, Hind III and Xho I restriction sites are underlined): 1CS (5′-at aagctt tggagcccgtggggcgcc-3′/5′-at ctcgag cgatgaacaaagagtcctg-3′), S (5′-at aagctt cacttggtgaagggcgac-3′/5′-at ctcgag cgatgaacaaagagtcctg-3′), and DC (5′-at aagctt ttgctagtcacggacccc-3′/5′-at ctcgag cgcaggtcttgccgtcccc-3′). .. The PCR products were digested with Hind III × Xho I and ligated in the Hind III × Xho I digested pSecTag 2B expression plasmid (ThermoFisher Scientific) using T4 DNA ligase (New England Biolabs). ..



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    90
    Thermo Fisher psectag 2b expression plasmid
    Psectag 2b Expression Plasmid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psectag+2b+expression+plasmid/psectag+2b+expression+plasmid/pmc08133003-78-15-25
    Average 90 stars, based on 1 article reviews
    psectag 2b expression plasmid - by Bioz Stars, 2026-09
    90/100 stars
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